| Date | Home | Away | Result |
|---|---|---|---|
| 21/02/25 19:30 | Sampdoria Genua | Sassuolo Calcio | 0-0 |
| 22/02/25 14:00 | AS Cittadella | Modena FC | 0-2 |
| 22/02/25 14:00 | AC Reggiana 1919 | Carrarese Calcio 1908 | 2-2 |
| 22/02/25 14:00 | AC Pisa | Juve Stabia | 3-1 |
| 22/02/25 14:00 | Mantova 1911 | SSC Bari | 0-1 |
| 22/02/25 16:15 | US Cremonese | FC Cesena | 1-2 |
| 23/02/25 14:00 | Brescia Calcio | FC Südtirol | --- |
| 23/02/25 14:00 | Cosenza Calcio | Palermo FC | --- |
| 23/02/25 14:00 | Spezia Calcio | US Catanzaro 1929 | --- |
| 23/02/25 16:15 | US Salernitana | Frosinone Calcio | --- |